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To obtain a highly active human liver UDP-glucuronosyltransferase, which conjugates several endogenous and xenobiotic substrates with glucuronic acid, an active cDNA clone encoding this enzyme was isolated from human liver. The cDNA contains an open reading frame of 1,485 nucleotides, encoding a protein of 491 amino acids (Mr = 57,518). This sequence is homologous with a number of glucuronosyltransferases of different species. The enzyme of human liver was expressed in Saccharomyces cerevisiae. This yeastsubunit was highly purified and was indistinguishable from the human liver enzyme with regard to its catalytic properties.Q:
Refactoring a giant python project?
My company is getting a large python project and it needs a lot of refactoring and cleaning up. I am an intermediate python programmer and I can only tell if it is a good approach to refactor a project: do a major restructuring in a sort of modular style (for example, implementing classes with similar features in separate classes), or do what I will do now: just comment out all the lines of code and keep only the comments. How can I choose between these two approaches?
A:
Documentation and automated tests are the other route to take, not “commenting out code and keeping the comments.” Code should be written with a comment at the top to explain the purpose of the code. When code does not meet that requirement, it is a bug. When it does, it is documentation and unit testing
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