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Simultaneous determination of dopamine, norepinephrine, epinephrine and 5-hydroxytryptamine in central nerve tissue by high-performance liquid chromatography with dual-electrode detection.
A simple and fast high-performance liquid chromatographic (HPLC) method with dual-electrode electrochemical detection for the simultaneous quantification of dopamine, norepinephrine, epinephrine and 5-hydroxytryptamine (serotonin) in central nervous tissue is described. The procedure consists of deproteinization with perchloric acid (0.1 N) followed by purification on a reversed-phase HPLC system using a macro-bore column (10 mm i.d. x 3.2 mm). Dopamine, norepinephrine, epinephrine and serotonin are separated within 10 min. The electrochemical detector set-up is based on two glassy carbon electrodes with different auxiliary electrolytes: one a 3-mm glassy carbon cathode and the other a 20-mm glassy carbon anode. Chromatographic resolution between neurotransmitters is good, and the detection limits are 2 microM for dopamine, norepinephrine and epinephrine, and 10 microM for serotonin. The reliability of the method for the quantification of central neurotransmitter levels was demonstrated by HPLC-electrochemical determination of catecholamine and serotonin levels in mouse brain in vivo.Current and historical results of venous thromboembolism prophylaxis in hip fracture surgery: a systematic review and meta-analysis.
There is a lack of consensus regarding the use of prophylactic anticoagulant/antiplatelet medications in hip fracture surgery. We performed a systematic review of all randomized controlled trials and meta-analyses comparing a drug (low-molecular-weight heparin [LMWH] or warfarin) with no drug for patients undergoing hip fracture surgery. A systematic literature search identified three meta-analyses and 26 randomized controlled trials. For all seven outcomes (deep venous thrombosis [DVT], pulmonary embolism [PE], major bleeding, mortality, thrombocytopenia, venous thromboembolism [VTE], and all-cause mortality), the results of three meta-analyses were
https://colab.research.google.com/drive/1WYt0ohm-WWETFGEGCRdpizjuqXNY_fDW
https://colab.research.google.com/drive/1EtkaRW1vgR-XL3cYC5Kg4WABndMoISwX
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https://colab.research.google.com/drive/1zz6flxPgyVXGggLmvp64Uyy8topoMbDA
https://colab.research.google.com/drive/1EjyY8lGt0T_xpr4zcbS_YIY05isOUsnP
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