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The generation of the outer membrane of Gram-negative bacteria like Neisseria meningitidis begins with the transfer of lipopolysaccharide (LPS) from the inner to the outer membrane through the periplasm. Lipid A (endotoxin) and LPS are important surface components of the meningococcus. Although lipid A plays a major role in prokaryotic and eukaryotic host response, the biological role of the meningococcal LPS (lipooligosaccharide or LOS) has not been fully characterized. In order to analyze the role of meningococcal LOS and LOS molecules from different LOS structures in the determination of the structure of the gram-negative bacterial outer membrane, the tetrasaccharide chains of six different MenB LOS types were isolated, purified, and studied in an ELISA system. The meningococcal serogroup B LOSs with the following structures: lipid A-IV (ChoP-II), lipid A-II (ChoP-III), lipid A-I (NeuP), lipid A-I (ChoP-I), lipid A-II (NeuP), and lipid A-II (ChoP-II) were found to be different in the immunoreactivity, the levels of endotoxin, and the immunogenicities of the tetra-saccharides from this study. These results support the idea that the distribution of LOS structures on the outer membrane is critical for the biological properties and the interaction with components of the host immune response. “I’m not… I was talking… about anything serious.” “We’re doing a thing.” “We really have to do this, otherwise it’s not gonna work.” “And it’s a total honour.” “I can tell you.” “It’s important to your mum.” “I won’t play with you if you don’t play with me.” “OK?” “Fine.” “How’s school going?” “Who
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