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Mechanisms underlying the diversity of osmo- and low-pH-induced gene expression were investigated in the cyanobacterium Synechocystis PCC 6803. A database of 990 genes differentially expressed under the control of two transmembrane osmotic stress-responsive promoters, PtlA1 and PtlA2, in a Synechocystis mutant in which DNA-binding activity of the cytotoxic factor OxyR is abolished, was constructed. There was a significant overlap between the gene expression programs induced in response to salt, Na(+) and K(+), and to the antifungal cyclic AMP-dependent protein kinase (cAPK) PCT1. The 681 genes that were induced by Na(+) and/or K(+) were strongly over-represented in genes coding for transporters or for ion-binding proteins, consistent with their role in maintaining the cellular concentration of the solutes. However, there were virtually no genes up-regulated by cAPK PCT1 in the absence of Na(+), K(+) or low pH. The PCT1 regulon was dominated by genes involved in nitrogen metabolism, whereas the most represented
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