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Installation is a matter of minutes. After the installation of the automatic balancing function, you can select the appropriate level of automatic adjustment. This is available as manual adjustment if this allows you to adapt the balancing result more closely to your individual requirements.Simultaneous determination of five active constituents in Angelicae dahuricae radix by a novel ultra performance liquid chromatography coupled with electrospray ionization quadrupole-time of flight mass spectrometry method.
A novel sensitive, rapid, accurate and easy to operate method for the simultaneous determination of five active constituents, namely daidzein (1), genistein (2), liquiritin (3), liquiritigenin (4) and isoliquiritin (5), in Angelicae dahuricae radix has been developed, involving ultrasonics-assisted extraction, followed by a novel ultra performance liquid chromatography coupled with electrospray ionization quadrupole-time of flight mass spectrometry (UPLC-ESI-QTOFMS) method. The LC-QTOFMS fingerprints of the five active constituents were established. Chromatographic separation was achieved on an Acquity UPLC® HSS T3 column with gradient elution using 0.1% (v/v) aqueous formic acid solution (A) and methanol (B) as the mobile phase, with a flow rate of 0.4 mL/min. The detection was conducted in positive and negative ion modes by multiple reaction monitoring (MRM) and selected reaction monitoring (SRM) modes, respectively. The UPLC method was successfully applied to the simultaneous determination of the five components in 24 batches of Angelicae dahuricae radix samples. The results showed that the novel method was rapid, accurate and sensitive for the simultaneous quantification of the five constituents in Angelicae dahuricae radix.
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